宫颈癌细胞瘤苗的主动免疫治疗研究

Experimental study of active specific immunotherapy for cervic cancer with tumor vaccine

摘要: 目的　研究添加新型佐剂的宫颈癌细胞肿瘤瘤苗的主动免疫治疗效果。方法　用Hela细胞重组白细胞介素 2、重组粒细胞单核细胞集落刺激因子、特制中药提取物及福氏不完全佐剂制成疫苗, 测定瘤苗免疫组、荷瘤组、正常组小鼠脾细胞宫颈癌细胞的杀伤活性 ;检测血清中IL 10、IFN γ水平 ;并计算存活期。结果　效靶比为 2 0 0 :1时, 免疫小鼠脾细胞体外杀伤宫颈癌细胞的杀伤率为 39%, 显著高于荷瘤组的14 %, 正常组的 8%。瘤苗免疫组血清IFN γ较其余组明显升高 (P <0 .0 1) ;血清IL 10较其余组明显降低(P <0 .0 1)。同时, 瘤苗免疫组生存期亦有明显延长。结论　新型肿瘤细胞瘤苗可激发特异性细胞介导的免疫反应, 改善抗肿瘤免疫反应

Abstract: Objectives To evaluate the active immunotherapeutic efficacy of tumor vaccine with new adjuvants. Methods HeLa cells, rIL-2,rGM-CSF,traditional Chinese medicine and Feud s adjuvant were mixed together to form tumor vaccine. An animal model of tumor vaccination was established. The 51 C r release assay was used to examine the cytotoxicities of three kinds of the splenocytes to cervic cancer cells. Serum IL-10, IFN-γ were tested. The other mice in every group were fed continously to calculate s...