反义PKCα对CNE-2Z细胞端粒酶活性的影响

Effect of protein kinase C-α antisense oligonucleotide on telomerase activity of CNE-2Z cells

摘要: 目的　观察反义PKCα对鼻咽癌CNE 2Z细胞生长及端粒酶活性的影响。方法　脂质体转染反义PKCα, MTT法检测细胞生长, TRAP ELISA法检测细胞端粒酶活性。结果　CNE 2Z细胞端粒酶活性为阳性, 转染反义PKCα可使细胞生长指数 (P <0 .0 1)及端粒酶活性 (P <0 .0 5 )降低。结论　反义PKCα可以抑制CNE 2Z细胞的生长及端粒酶活性, PKCα可能通过调控端粒酶活性影响CNE 2Z细胞的生长。

Abstract: Objective To observe the effect of protein kinase c-α (PKCα)antisense oligonucleotide on cell growth and telomerase activity in human poor-differentiated nasopharyngeal carcinoma(NPC) cell line CNE-2Z. Methods Antisense PKCα was transfected by cationic liposome in CNE-2Z cells to analyze the cell growth and telomerase activity by MTT colorimetric assay and TRAP-ELISA,respectively. Results The telomerase activity was positive in CNE-2Z.After treated with antisense PKCα, the relative cell growth index...