Abstract: Objective 　To investigate the expression, clinical significance and relationship of phospho-AKT (p-A KT), AKT2 and PTEN in human non-small cell lung cancer (NSCLC) tissue. Methods 　The expression of p-AKT, AKT2 and PTEN in 80 cases of NSCLC and 35 cases of no-cancerous lung disease were assessed by immunohistochemistry, and their correlations with clinicopathologic factors were statistically analyzed. Results 　The positive rate of p-AKT, AKT2 was 78. 8 %、91. 3 % in NSCLC and 0 %、5. 7 % ( P < 0. 05) in no-cancerous lung disease. But the positive rate of PTEN (47. 5 %) was significantly lower than that of PTEN (94. 3 %) in no-cancerous lung disease ( P < 0. 05) . The expression of p-AKT didn't relate to age, sex, histological subtype and tumor differentiation, lymph node metastasis and TNM stages ( P > 0. 05) . The positive rate of AKT2 in the group with lymph node metastasis (100. 0 %) was significantly higher than that of AKT2 in the group without lymph node metastasis (80. 6 %) ( P <0. 05) . The expression of PTEN correlated with lymph node metastasis and differentiation of NSCLC ( P< 0. 05) . In addition, Positive correlations were observed between the expression of AKT2 and p-A KT in NSCLC tissues ( P < 0. 05) . The expression of PTEN had negative correlations with the expression of p-A KT and AKT2 ( P < 0. 05) . Conclusion 　A KT activation may be present in NSCLC. The loss of expression of PTEN may correlate to activation of AKT. AKT2 may be one of regulated forms of PTEN.