Abstract: Objective To evaluate the toxicity and efficacy of combination chemotherapy with paclitaxel plus cisplatin and fluorouracil(PCF)in advanced gastric cancer. Methods Between 2002 and 2006,thirty-five advanced gastric cancer patients who received PCF regimen were analyzed retrospectively.The combination chemotherapy included paclitaxel 150 mg/m2 as 3 hours infusion on day 1,cisplatin 12 mg/m2/d on days 1 to 5 and 5-Fu 3 g/m2 over 120h infusion.The therapy repeated every 3 weeks. Results Fifteen local advanced gast ric cancer patient s received PCF regimen af ter operations. Median time to progression and overall survival time were 11. 0 months and 18. 6 months, respectively. Twenty patient s with metastatic gast ric cancer received PCF regimen as salvage therapy. Fif teen patient s were assessable. Complete response 0 (0), partial response 3 (20 %), stable disease 9 (60 %) and progressive disease 3 (20 %) . Median time to progression and overall survival time were 7. 0 months and 9. 0 months, respectively. Frequent grade 3/ 4 toxicity for PCF were :nausea 18. 1 %, neut ropenia 9. 4 % and fatigue 7. 9 %. Febrile neut ropenia occurred 9. 5 %. Conclusion 　The toxicity of PCF regimen was acceptable and the efficacy needed further evaluation.