Abstract: Objective 　To explore the expression feature and significance of heat shock protein 70 and p53 protein in human colorectal carcinoma 、para-tumor tissue and normal tissue . Methods 　SP immunohisto-chemical technique and image analysis were used . Results 　① In a total of 31 cases, the average value of light density of HSP70 were 0. 5917 ±0. 0678, 0. 3363 ±0. 0485, 0. 1742 ±0. 0193 and p53 proteins were 0. 7547 ±0. 2732, 0. 5388 ±0. 1542, 0. 3770 ±0. 0682 respectively ; A high expression of HSP70 and p53 protein were discovered and showed a significant difference ( P < 0. 01) in tumor tissue compared with para-tumor tissue and normal tissue ; ② The expression of HSP70 and p53 protein were a linear correlation and the correlation coefficient ( r) of them was 0. 795. There was a positive correlation between the expression of HSP70 and p53 protein in human colorectal carcinoma. Conclusion 　① The result s suggested that HSP70 and p53 protein might play an important role in the development of human colorectal carcinoma, and the result was able to a malignant index in cytology. ②HSP70 and p53 protein might has a coordinated effect in the course of generation and development in human colorectal carcinoma, and might be associated with HSP70 and p53 protein compound form existence in human colorectal tumor tissue probably.