阿霉素加热化疗对抗人肝癌耐药细胞多药耐药性的实验研究

Studies on the Confrontation of Thermo-chemotherapy of ADM on Human Multidrug -resistant Hepatocellular Carcinoma Cell Line

摘要: 目的　观察比较阿霉素 ( ADM)化疗与 43℃加热化疗对耐药人肝癌细胞模型 - 772 1 /Adm(以下简称 772 1 /Adm细胞 )的敏感性及细胞内药物浓度的影响。方法　以人肝癌细胞模型 -772 1 /Adm为研究对象, 采用水浴加温法、体外细胞毒试验 ( MTT法 )、流式细胞技术, 观察阿霉素( ADM)化疗与加热化疗后细胞的存活率及细胞内阿霉素浓度的变化。结果　 ( 1 )阿霉素化疗、加热化疗 30、60 min后 772 1 /Adm细胞存活率分别为 70 .2 %、40 .8%和 60 .2 %、37.4% ;( 2 )流式细胞仪检测显示阿霉素化疗、加热化疗 30 min后细胞内阿霉素浓度分别为 41 .3%、92 .0 %。结论　加热可以显著对抗 772 1 /Adm的耐药性, 提高其对阿霉素的敏感性, 这与加热提高了细胞内药物浓度有关。

Abstract: Objective To comparatively observe the effects of thermo-chemotherapy of ADM on human multidrug-resistant hepatocellular carcinoma cell line (7721/Adm) to sensitivity and intracellular concentration of ADM. Methods By means of water bath and MTT assay,the sensitivity of 7721/Adm to ADM was observed,and the effects of hyperthermia on intracellular concentration of ADM of 7721/Adm was detected by flow cytometry. Results (1) Cell line survive rates of 7721/Adm were 70.2%?40.8% after chemotherapy and thermo-che...