HSVTK/GCV自杀基因系统治疗乳腺癌的研究
A Study of the RV-HSV-TK/GCV Suicide Gene Therapy System in Breast Carcinoma
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摘要: 目的 探讨HSV TK/GCV自杀基因系统对小鼠乳腺癌细胞系MA782 / 5S 810 2体外及体内杀伤作用及其产生的旁观者效应。方法 采用脂质体转染法将GINaTK载体转入包装细胞PA317。取病毒上清液感染小鼠乳腺癌细胞MA782 / 5S 810 2, 得到带有HSV TK基因的MA782 / 5S 810 2 /TK细胞, 并将其分别用于体外和体内实验。结果 载体HSV TK导入了PA317细胞。体外实验结果显示, 当MA782 / 5S 810 2 /TK细胞数占混合细胞 10 %时, 低浓度 (10 μg/ml)的GCV就可将 5 0 %左右的肿瘤细胞杀死。体内实验结果显示GCV可明显抑制MA782 / 5S 810 2 /TK细胞在BALB/C小鼠体内的肿瘤形成。实验组肿瘤组织与对照组相比存在明显的病理学改变。结论 逆转录病毒可介导HSV TK基因转入小鼠乳腺癌细胞MA782 / 5S 810 2并获稳定表达, HSV TK/GCV自杀基因系统在体内外对乳腺癌细胞均有杀伤作用, 且存在明显的旁观者效应。Abstract: Objective To study the killing effect in vitro and in vivo and the bystander effect of HSV-TK/GCV suicide gene system on mice breast carcinoma cells MA782/5S-8102. Methods GINaTK retroviral vector containing HSV-TK gene was transduced into PA317 packaging cell by lipofectin.Mice breast carcinoma cell line MA782/5S-8102 was infected by high titer viral supernatate. PCR Was resorted to demonstrate the successful transduction of the HSV-TK gene. MA782/5S-8102 /TK cells and MA782/5S-8102 cells were ...
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