环状RNA hsa_circ_0018574在结直肠癌组织中的表达及其对结直肠癌细胞增殖的影响

马锐; 田进海; 马荣; 万巧凤; 刘河涛; 王立斌

doi:10.3971/j.issn.1000-8578.2022.22.0516

环状RNA hsa_circ_0018574在结直肠癌组织中的表达及其对结直肠癌细胞增殖的影响

Expression of Circular RNA hsa_circ_0018574 in Colorectal Cancer Tissues and Its Effect on Proliferation of Colorectal Cancer Cells

摘要

摘要:
目的探讨hsa_circ_0018574在结直肠癌组织和人结肠癌HT29细胞系中的表达，以及其对结直肠肿瘤细胞增殖和凋亡的影响。
方法使用circPrimer1.2软件绘制circRNA序列结构示意图，用人circRNA芯片筛选在结直肠癌组织和癌旁组织中差异表达的circRNA，提取组织样本RNA，RT-qPCR检测hsa_circ_0018574在人结直肠肿瘤组织中的表达。将si-circ_0018574转染至人结肠癌HT29细胞，分别采用克隆集落形成实验、流式细胞仪、Western blot法检测CDK2、CDK4、CDK6以及cyclinD1和cyclinE周期蛋白表达。
结果 hsa_circ_0018574在人结直肠肿瘤组织中的表达明显高于癌旁组织（P < 0.01）。si-circ_0018574能够明显抑制人结肠癌HT29细胞增殖，减少克隆形成以及降低细胞集落的形成能力（P < 0.01），诱导肿瘤细胞凋亡（P < 0.01）。CDK2、CDK4、CDK6以及cyclinD1和cyclinE周期蛋白表达降低。
结论 hsa_circ_0018574在结直肠肿瘤中高表达，沉默circ_0018574能够显著抑制人结肠癌HT29细胞增殖，减少细胞分裂，诱导细胞凋亡。

Abstract:
Objective To investigate the expression of hsa_circ_0018574 in colorectal cancer tissues and human colon cancer HT29 cell line, as well as its effect on the proliferation and apoptosis of colorectal cancer cells.
Methods The circPrimer 1.2 software was used to draw the circRNA sequence structure. Meanwhile, the circRNA microarray was used to screen differentially-expressed circRNA in colorectal cancer tissues and adjacent tissues, and RNA was extracted from tissue samples. The expression of hsa_circ_0018574 in human colorectal tumors was detected by RT-qPCR. The si-circ_0018574 was transfected into HT29 cells, and the expression of CDK2, CDK4, CDK6, cyclinD1, and cyclinE cyclins were detected by colony formation assay, flow cytometry, and Western blot, respectively.
Results The expression of hsa_circ_0018574 in human colorectal tumor tissues was significantly higher than that in adjacent tissues (P < 0.01). Meanwhile, the si-circ_0018574 in HT29 cells could significantly inhibit the proliferation of cancer cells, reduce clone formation and colony formation ability (P < 0.01), and induce tumor cell apoptosis (P < 0.01). The expressions of CDK2, CDK4, CDK6, cyclinD1 and cyclinE cyclins were decreased.
Conclusion The hsa_circ_0018574 is highly expressed in colorectal tumors, and si-circ_0018574 could significantly inhibit the proliferation of HT29 cells, reduce cell division, and induce apoptosis.

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