miR-581调控FOXO1对卵巢癌SKOV3细胞自噬的影响

高海宁; 白瑞霞; 赵鹏伟; 宋婉莹; 林萱

doi:10.3971/j.issn.1000-8578.2022.21.1202

miR-581调控FOXO1对卵巢癌SKOV3细胞自噬的影响

Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1

摘要

摘要:
目的探讨miR-581对卵巢癌SKOV3细胞自噬的影响及作用机制。
方法将miR-581 mimics和miR-581 NC转染到卵巢癌SKOV3细胞中，实时荧光定量PCR法（qRT-PCR）检测转染效率；转染成功后，Western blot检测转染后卵巢癌SKOV3细胞中自噬相关蛋白的表达水平；TargetScanHuman数据库预测miR-581靶基因，Western blot验证miR-581与靶基因的作用。
结果过表达miR-581后可显著抑制自噬相关蛋白LC3Ⅱ和Beclin1的表达（P < 0.01）；miR-581可负向调控FOXO1的表达（P < 0.01）；FOXO1促进卵巢癌SKOV3细胞自噬（P < 0.05）。
结论 miR-581通过调控FOXO1的表达影响卵巢癌SKOV3细胞自噬。

Abstract:
Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells.
Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes.
Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3 Ⅱ and Beclin1 (P < 0.01); miR-581 negatively regulated FOXO1 expression (P < 0.01) and FOXO1 promoted autophagy of SKOV3 cells (P < 0.01).
Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1.

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