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文中驰, 刘拓宙, 何洪波, 章灿, 刘育鹏, 廖瞻, 曾莉怡. IGF1受体的β亚基突变体对人骨肉瘤细胞增殖、迁移和凋亡的影响[J]. 肿瘤防治研究, 2022, 49(5): 390-395. DOI: 10.3971/j.issn.1000-8578.2022.21.1104
引用本文: 文中驰, 刘拓宙, 何洪波, 章灿, 刘育鹏, 廖瞻, 曾莉怡. IGF1受体的β亚基突变体对人骨肉瘤细胞增殖、迁移和凋亡的影响[J]. 肿瘤防治研究, 2022, 49(5): 390-395. DOI: 10.3971/j.issn.1000-8578.2022.21.1104
WEN Zhongchi1, LIU Tuozhou1, HE Hongbo, ZHANG Can, LIU Yupeng, LIAO Zhan, ZENG Liyi. Effect of IGF1Rβ Subunit Mutants on Proliferation, Migration and Apoptosis of Human Osteosarcoma Cells[J]. Cancer Research on Prevention and Treatment, 2022, 49(5): 390-395. DOI: 10.3971/j.issn.1000-8578.2022.21.1104
Citation: WEN Zhongchi1, LIU Tuozhou1, HE Hongbo, ZHANG Can, LIU Yupeng, LIAO Zhan, ZENG Liyi. Effect of IGF1Rβ Subunit Mutants on Proliferation, Migration and Apoptosis of Human Osteosarcoma Cells[J]. Cancer Research on Prevention and Treatment, 2022, 49(5): 390-395. DOI: 10.3971/j.issn.1000-8578.2022.21.1104

IGF1受体的β亚基突变体对人骨肉瘤细胞增殖、迁移和凋亡的影响

Effect of IGF1Rβ Subunit Mutants on Proliferation, Migration and Apoptosis of Human Osteosarcoma Cells

  • 摘要:
    目的  探讨IGF1受体(IGF-1R)的β亚基突变体(sb-IGF1R、ma-IGF1R)对骨肉瘤143B细胞生物学行为的影响。
    方法  设计与构建sb-IGF1R、ma-IGF1R片段,克隆到腺病毒AdEasy穿梭质粒中,获得Ad-sbIGF1R、Ad-maIGF1R,再将两者与Ad-GFP分别转染骨肉瘤细胞143B,观察细胞的增殖、迁移及凋亡情况;构建Ad-sbIGF1R、Ad-maIGF1R及Ad-GFP裸鼠皮下荷瘤模型,评价肿瘤在体外的生长情况。
    结果  通过质粒PCR验证,IGF-1Rβ亚基突变体在骨肉瘤细胞中过表达。与对照组相比,经Ad-sbIGF1R、Ad-maIGF1R处理过的骨肉瘤细胞,其细胞增殖、迁移能力显著抑制,促进了骨肉瘤细胞的凋亡。同时在裸鼠皮下荷瘤模型中抑制肿瘤生长。
    结论  IGF1受体的β亚基突变体抑制骨肉瘤细胞增殖和转移,并促进骨肉瘤细胞凋亡。

     

    Abstract:
    Objective  To investigate the effect of IGF1R β subunit mutants sb-IGF1R and ma-IGF1R on the biological behavior of osteosarcoma 143B cells.
    Methods  We designed and constructed sb-IGF1R and ma-IGF1R fragments. They were cloned into adenovirus AdEasy shuttle plasmid, to obtain Ad-sbIGF1R and Ad-maIGF1R. We observed the proliferation, migration and apoptosis of the osteosarcoma cells transfected with Ad-sbIGF1R, Ad-maIGF1R and Ad-IGF1R. The Ad-sbIGF1R, Ad-maIGF1R and Ad-GFP nude mouse models were constructed to evaluate the tumor growth in vitro.
    Results  By plasmid PCR, IGF-1R β subunit mutant was overexpressed in osteosarcoma cells. Ad-sbIGF1R and Ad-maIGF1R significantly inhibited the proliferation and migration of osteosarcoma cells, and promoted cell apoptosis, and inhibited tumor growth in subcutaneous tumor-bearing nude mouse models.
    Conclusion  IGF1R β subunit mutants inhibit the proliferation and migration of osteosarcoma cells and induce cell apoptosis.

     

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