血管内皮细胞中RhoC的表达对骨髓瘤RPMI8226细胞增殖和侵袭的影响

孙淼淼; 刘凯; 王瞳; 邱森; 赵志华; 陈奎生

doi:10.3971/j.issn.1000-8578.2022.21.0934

血管内皮细胞中RhoC的表达对骨髓瘤RPMI8226细胞增殖和侵袭的影响

Effect of RhoC Expression in Vascular Endothelial Cells on Proliferation and Invasion of Myeloma RPMI8226 Cells

摘要

摘要:
目的探讨RhoC在血管内皮细胞中表达对骨髓瘤RPMI8226细胞增殖、侵袭能力的影响及可能机制。
方法构建RhoC shRNA慢病毒载体转染骨髓瘤血管内皮细胞（MVECs）和人脐静脉内皮细胞（HUVECs），分别采用CCK-8试剂盒、流式细胞仪、Transwell实验检测条件培养基对骨髓瘤RPMI8226细胞增殖、细胞周期和侵袭能力的影响；Western blot法检测CDK、CyclinD1、AKT、PI3K、MMP2、MMP9等蛋白表达。
结果下调MVECs和HUVECs中RhoC表达，RhoC shRNA组骨髓瘤RPMI8226细胞增殖和侵袭能力均较阴性对照组降低，细胞周期阻滞在G₀/G₁期（P < 0.05）。RhoC shRNA组CDK、CyclinD1、AKT、PI3K、MMP2、MMP9等蛋白表达均较阴性对照组降低（P < 0.05）。
结论 RhoC在MVECs中表达，对骨髓瘤RPMI8226细胞增殖和侵袭能力具有调节作用；其机制可能与CDK、CyclinD1、AKT、PI3K、MMP2、MMP9等参与有关。

Abstract:
Objective To investigate the effect of RhoC expression in vascular endothelial cells on the proliferation and invasion of myeloma RPMI8226 cells and its possible mechanism.
Methods RhoC shRNA lentivirus vector was constructed and transfected into myeloma vascular endothelial cells (MVECs) and human umbilical vein endothelial cells (HUVECs). The effects of conditioned medium on the proliferation, cell cycle and invasion of RPMI8226 cells were detected by CCK-8 test, flow cytometry and Transwell test. The expression of CDK, CyclinD1, AKT, PI3K, MMP2 and MMP9 were detected by Western blot.
Results The expression of RhoC in MVECs and HUVECs were downregulated. The proliferation and invasion of RPMI8226 cells in RhoC shRNA group were lower than those in negative control group, and the cell cycle was blocked in G₀/G₁ phase (P < 0.05). The expressions of CDK, CyclinD1, AKT, PI3K, MMP2 and MMP9 in RhoC shRNA group were lower than those in negative control group (P < 0.05).
Conclusion The expression of RhoC in MVECs can regulate the proliferation and invasion of myeloma RPMI8226 cells, and the mechanism may be related to the participation of CDK, CyclinD1, AKT, PI3K, MMP2 and MMP9.

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