横纹肌肉瘤组织中JMJD2B和HIF-1α的表达及其对预后的意义

吴宏增; 赵嘉政; 赵祎; 马晓伟; 冯和林

doi:10.3971/j.issn.1000-8578.2021.21.0030

横纹肌肉瘤组织中JMJD2B和HIF-1α的表达及其对预后的意义

Expression of JMJD2B and HIF-1α in Rhabdomyosarcoma Tissues and Their Prognostic Significance

摘要

摘要:
目的探讨组蛋白去甲基化酶JMJD2B和HIF-1α在横纹肌肉瘤（RMS）组织中的表达及意义。
方法选取78例RMS患者通过随访获取生存时间，采用免疫组织化学染色法检测JMJD2B和HIF-1α在RMS组织中的表达情况并分析其与患者临床病理特征及预后的关系。
结果 78例样本中，JMJD2B和HIF-1α表达阳性例数分别为46例（59.0%）和44例（56.4%）。JMJD2B和HIF-1α的表达成正相关（P < 0.001, r=0.518）且与肿瘤的危险度、治疗、转移、分期有关（均P < 0.05)。生存分析显示危险度的级别、新辅助化疗的有无、HIF-1α和JMJD2B表达的高低与患者预后相关（均P < 0.05）。多因素Cox回归分析进一步显示JMJD2B（HR=3.161, P=0.039）和危险度（HR=2.925, P=0.001）可作为RMS患者独立的预后因素。
结论 JMJD2B和HIF-1α的表达与横纹肌肉瘤的总体生存密切相关，JMJD2B是横纹肌肉瘤患者的独立预后指标。

Abstract:
Objective To explore the expression and prognostic significance of histone demethylase JMJD2B and HIF-1α in rhabdomyosarcoma(RMS) tissues.
Methods A total of 78 RMS patients were followed up to obtain survival time, and immunohistochemical staining was used to detect the expression of JMJD2B and HIF-1α in RMS tissues and analyze their relation with clinicopathological characteristics and prognosis.
Results Among the 78 samples, there were 46 (59.0%) and 44 (56.4%) cases of positive expression of JMJD2B and HIF-1α, respectively. The expression of JMJD2B and HIF-1α were positively correlated (P < 0.001, r=0.518) and related to tumor risk, metastasis, IRS staging and neoadjuvant chemotherapy (all P < 0.05). The Kaplan-Meier survival curve and univariate Cox regression analysis showed that the risk level, neoadjuvant chemotherapy, the expression of HIF-1α and JMJD2B were related to the prognosis of patients (all P < 0.05). Multivariate Cox regression analysis showed that JMJD2B (HR=3.161, P=0.039) and risk degree (HR=2.925, P=0.001) could be used as independent prognostic factors for RMS patients.
Conclusion JMJD2B and HIF-1α expression are both significantly related to the overall survival of rhabdomyosarcoma patients, and JMJD2B is an independent prognostic indicator.

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