丹参水溶性组分SABP对小鼠肝癌免疫微环境的影响

李圣豪; 郝立园; 国英琳; 彭晴; 丁静茹; 石新丽

doi:10.3971/j.issn.1000-8578.2021.20.1405

丹参水溶性组分SABP对小鼠肝癌免疫微环境的影响

Effect of SABP, A Water-soluble Component of Salvia Miltiorrhiza, on Immune Microenvironment of Mice with Liver Cancer

摘要

摘要:
目的探讨丹参水溶性组分SABP影响H22细胞肝癌原位移植瘤生长及肝癌免疫微环境的效应。
方法建立H22细胞肝癌原位移植瘤小鼠模型。ELISA检测肝脏中PD-L1、TGF-β、IL-1β、IL-10、IL-4、IFN-γ、IL-18、IL-7、IL-2、CCL-2和CCL-21的表达量。计算肝脏、脾脏和肾脏的脏器系数。
结果 SABP抑制H22细胞肝癌原位移植瘤的生长，提高肝癌微环境中PD-L1、TGF-β、IL-1β和IL-10的表达水平，增加了肝、脾和肾系数。
结论 SABP能抑制H22细胞肝癌原位移植瘤生长，促进肝癌微环境中PD-L1、TGF-β、IL-1β和IL-10抑制性免疫分子的表达。

Abstract:
Objective To investigate the effect of SABP, a water-soluble component of Salvia miltiorrhiza, on the growth of orthotopic transplantation of H22 liver cancer and the immune microenvironment of liver cancer.
Methods We established a mouse model of orthotopic transplantation of H22 cell liver cancer in BALB/c mice. ELISA was used to detect the expression of PD-L1, TGF-β, IL-1β, IL-10, IL-4, IFN-γ, IL-18, IL-7, IL-2, CCL-2 and CCL-21 in the liver. We counted the organ indexes of liver, spleen and kidney.
Results SABP inhibited the growth of orthotopic transplantation tumors of H22 cell liver cancer, and increased the expression levels of PD-L1, TGF-β, IL-1β and IL-10 in the microenvironment of liver cancer, as well as the liver, spleen and kidney coefficients.
Conclusion SABP could inhibit the growth of orthotopic transplantation tumors of H22 cell liver cancer and promote the expression of PD-L1, TGF-β, IL-1β and IL-10 in the microenvironment of liver cancer.

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