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宁佳羽, 包伟晶, 周素娟, 魏日富, 朱忠寿. LncRNA-MEG3和KLF4在鼻咽癌发生发展中的作用及可能机制[J]. 肿瘤防治研究, 2021, 48(3): 234-238. DOI: 10.3971/j.issn.1000-8578.2021.20.0790
引用本文: 宁佳羽, 包伟晶, 周素娟, 魏日富, 朱忠寿. LncRNA-MEG3和KLF4在鼻咽癌发生发展中的作用及可能机制[J]. 肿瘤防治研究, 2021, 48(3): 234-238. DOI: 10.3971/j.issn.1000-8578.2021.20.0790
NING Jiayu, BAO Weiijing, ZHOU Sujuan, WEI Rifu, ZHU Zhongshou. Role and Possible Mechanism of LncRNA-MEG3 and KLF4 in Carcinogenesis and Progression of Nasopharyngeal Carcinoma[J]. Cancer Research on Prevention and Treatment, 2021, 48(3): 234-238. DOI: 10.3971/j.issn.1000-8578.2021.20.0790
Citation: NING Jiayu, BAO Weiijing, ZHOU Sujuan, WEI Rifu, ZHU Zhongshou. Role and Possible Mechanism of LncRNA-MEG3 and KLF4 in Carcinogenesis and Progression of Nasopharyngeal Carcinoma[J]. Cancer Research on Prevention and Treatment, 2021, 48(3): 234-238. DOI: 10.3971/j.issn.1000-8578.2021.20.0790

LncRNA-MEG3和KLF4在鼻咽癌发生发展中的作用及可能机制

Role and Possible Mechanism of LncRNA-MEG3 and KLF4 in Carcinogenesis and Progression of Nasopharyngeal Carcinoma

  • 摘要:
    目的 探讨LncRNAs-MEG3在鼻咽癌发生发展中的作用及其可能的分子机制。
    方法 qRT-PCR检测鼻咽癌细胞中MEG3与miR-543的含量,荧光素酶报告法研究MEG3与miR-543的关系,分析MEG3和KLF4介导的鼻咽癌细胞增殖和凋亡的变化,Western blot检测KLF4、Bcl-2和Bax蛋白表达水平。
    结果 鼻咽癌细胞株中MEG3的表达呈下降趋势,miR-543的表达呈上升趋势,与对照组相比差异有统计学意义(P < 0.05);荧光素酶报告和Western blot显示MEG3可结合miR-543以调控KLF4的表达,进而抑制肿瘤细胞增殖、促进肿瘤细胞凋亡并影响Bcl-2和Bax蛋白表达水平,与对照组相比差异有统计学意义(P < 0.05)。
    结论 LncRNA-MEG3可通过吸附结合miR-543靶向调控KLF4的表达,发挥抑制鼻咽癌发生发展的作用。LncRNA-MEG3可能是鼻咽癌靶向治疗的一种新的生物标志物。

     

    Abstract:
    Objective To investigate the role of LncRNAs-MEG3 in the carcinogenesis and progression of nasopharyngeal carcinoma (NPC) and the possible molecular mechanism.
    Methods qRT-PCR was used to detect the content of MEG3 and miR-543 in NPC cells. Luciferase reporter method was used to study the relation between MEG3 and miR-543, and the changes of cell proliferation and apoptosis induced by MEG3 or KLF4 were analyzed. Western blot was used to detect the expression of KLF4, Bcl-2 and Bax proteins.
    Results Compared with the control group, the expression of miR-543 in NPC cell line was significantly increased (P < 0.05), while the expression of MEG3 was decreased (P < 0.05). Luciferase report and Western blot showed that MEG3 could regulate the expression of KLF4 by adsorbing miR-543 to inhibit cell proliferation, promote cell apoptosis and affect the expression levels of Bcl-2 and Bax proteins.
    Conclusion LncRNA-MEG3 could regulate the expression of KLF4 by adsorbing miR-543 and then plays a role in inhibiting the occurrence and development of NPC. It may be a new biomarker for NPC targeted therapy.

     

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