三氧化二砷对阿霉素耐药的白血病细胞血管新生相关因子的影响

Effect of Arsenic Trioxide on Related Factors of Angiogenesis in K562/A02 Cells

摘要: 目的探讨三氧化二砷（arsenictrioxide,As2O3）对白血病耐药细胞株K562/A02细胞血管新生相关因子血管内皮生长因子（VEGF）表达水平及基质金属蛋白酶2、9（MMP-2、9）活性的影响。方法用噻唑蓝法（MTT）测定As2O3对K562/A02细胞的增殖抑制作用,用酶联免疫吸附试验测定细胞上清中VEGF含量,用明胶酶谱法半定量测定细胞上清中MMP-2、9的活性。结果 K562/A02细胞的IC50是（1.64±0.16）μM,0.05μMAs2O3对细胞增殖和VEGF的表达没有明显影响,对MMP-2、9的活性在24、48h无明显影响（P〉0.05）,但在72h则对MMP-2、9的活性有明显抑制作用（P〈0.05）;0.4和3.2μMAs2O3可显著抑制细胞增殖,下调VEGF并使MMP-2、9活性减弱（P〈0.05）。结论 As2O3可能通过下调VEGF的表达和抑制MMP-2、9活性而发挥抗白血病细胞血管新生作用。

Abstract: Objective To investigate the effect of arsenic trioxide (As2O3) on the expression of VEGF and the activity of MMP-2 and 9 in K562/A02 cells. Methods MTT assay was used to detect the inhibition ratio of K562/A02 cell. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of VEGF. Gelatin zymography assay was used to assess the activity of MMP-2, 9. Results Neither the proliferation nor VEGF expression of K562/A02 cells was influenced by 0.05 μM As2O3 after 24, 48 and 72 hours' incubation. Th...