脑胶质瘤中CXC趋化因子表达的预后意义及免疫细胞浸润分析

刘芳远; 苏秀兰

doi:10.3971/j.issn.1000-8578.2020.21.0435

脑胶质瘤中CXC趋化因子表达的预后意义及免疫细胞浸润分析

Prognostic Significance and Immune Cell Infiltration Analysis of CXC Chemokines in Glioblastoma

摘要

摘要:
目的分析多形性胶质母细胞瘤(GBM)中CXC趋化因子的表达，研究其在GBM中的预后价值及其作为治疗靶点的潜在作用。
方法利用GEPIA数据库筛选出差异表达的CXC趋化因子，并分析CXC趋化因子对GBM患者预后的影响。使用String数据库预测邻近基因，并构建蛋白互作网络(PPI)。使用DAVID数据库进行GO富集分析和KEGG通路富集分析。使用TRRUST和LinkedOmics预测转录因子和激酶靶标。使用TIMER分析差异表达趋化因子与肿瘤纯度和免疫细胞浸润程度的相关性。
结果 CXCL2/3/8/9/10/11/16在GBM中的表达显著上调，其中CXCL3/8与患者不良预后显著相关。差异表达趋化因子及其邻近基因主要富集于免疫调控和细胞凋亡的功能和信号通路。趋化因子的表达与六种免疫细胞的浸润水平存在相关性。
结论 CXCL3/8可作为GBM患者潜在的预后标志物。CXC趋化因子与GBM肿瘤免疫有关。

Abstract:
Objective To analyze the expression of CXC chemokines in glioblastoma (GBM), and investigate their prognostic value and potential roles as therapeutic targets.
Methods Differential expression and prognostic value of CXC chemokines in GBM were obtained in GEPIA. The String database was used to predict neighbor genes and construct the protein to protein interaction (PPI) network. GO analysis and KEGG pathway enrichment analysis were executed by DAVID tools. TRRUST and LinkedOmics were used to predict transcription factor and kinase targets for CXC chemokines. Finally, we discussed the correlation of immune cell infiltration and tumor purity with differential expression of CXC chemokines in GBM.
Results CXC2/3/8/9/10/11/16 expression was significantly up-regulated in GBM, and CXC3/8 were significantly associated with the poor prognosis of GBM patients. Differentially expressed chemokines and their adjacent genes are mainly enriched in the immune regulatory and apoptosis functions and pathways. There was correlation between chemokines expression and infiltration of six immune cells.
Conclusion CXCL3/8 could be the potential prognostic markers for GBM patients. CXC chemokines are related to GBM immunity.

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