抑制DLK1表达对食管癌细胞放射敏感度的影响

何翠; 陈春丽; 柯青; 沈力; 邓鑫州; 顾兵; 孙蕊格; 段奇文

doi:10.3971/j.issn.1000-8578.2020.19.1093

抑制DLK1表达对食管癌细胞放射敏感度的影响

Effect of Inhibiting DLK1 Expression on Radiosensitivity of Esophageal Cancer Cells

摘要

摘要:
目的探讨抑制DLK1的表达对食管癌细胞放射敏感度的影响。
方法采用克隆形成实验验证KYSE-R与KYSE细胞的放射敏感度差异，RT-PCR和Western blot检测DLK1的表达。选用最佳DLK1干扰序列转染KYSE-R细胞，4 Gy射线处理后克隆形成实验检测KYSE-R细胞放射敏感度，流式细胞术检测细胞凋亡和细胞周期。
结果 KYSE-R细胞表现出比KYSE细胞更强的放射抗拒性，DLK1在KYSE-R中的表达显著高于KYSE，且siDLK1-3干扰效果最佳（均P < 0.05）。4 Gy射线照射后DLK1干扰组细胞存活力显著低于对照组，而DLK1干扰组细胞凋亡率和G₂/M期细胞比例明显高于对照组（均P < 0.05）。
结论抑制DLK1表达可通过降低细胞存活、促进细胞凋亡和增加G₂/M期细胞比例、增强放射敏感度，为食管癌放射增敏研究提供新的靶点。

Abstract:
Objective To investigate the effect of inhibiting DLK1 expression on the radiosensitivity of esophageal cancer cells.
Methods The difference in radiosensitivity between KYSE-R and KYSE cells was verified by colony formation assay. RT-PCR and Western blot were used to detect DLK1 expression. The best DLK1 interference sequence was used to transfect KYSE-R cells. After 4Gy radiation treatment, clone formation experiment was used to detect the radiosensitivity of KYSE-R cells, and flow cytometry was used to detect apoptosis and cell cycle.
Results KYSE-R cells showed stronger radioresistance than KYSE cells. The expression of DLK1 in KYSE-R cells was significantly higher than that in KYSE cells, and the interference effect of siDLK1-3 was the best(all P < 0.05). Compared with the control group, the cell viability was significantly lower after 4Gy irradiation, while the apoptosis rate and cell proportion in G₂/M phase were significantly higher in DLK1 interference group(all P < 0.05).
Conclusion The inhibition of DLK1 expression could provide a new target for radiosensitization research of esophageal cancer by reducing cell survival, promoting apoptosis, increasing the cell proportion in G₂/M phase and enhancing radiosensitivity.

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