腺病毒介导的Hes1基因抑制肝细胞癌细胞的增殖与迁移

于兵; 尹刚; 胡以平

doi:10.3971/j.issn.1000-8578.2020.19.0756

腺病毒介导的Hes1基因抑制肝细胞癌细胞的增殖与迁移

Adenovirus-mediated Hes1 Expression Inhibits Proliferation and Migration of Hepatocellular Carcinoma Cells

摘要

摘要:
目的探讨Hes1基因对肝细胞癌细胞系Hep1-6细胞增殖、迁移与侵袭的影响。
方法通过重组腺病毒载体介导Hes1基因在Hep1-6细胞系中过表达，随后测定细胞克隆形成率、增殖速率及体外迁移与侵袭能力的改变。
结果 Hep1-6细胞分别感染Ad-Hes1和阴性对照Ad-GFP后，感染Ad-Hes1的细胞系克隆形成数显著少于对照组（P < 0.001）；感染病毒6天后，CCK-8检测感染Ad-Hes1的细胞系在OD_{450 nm}的吸光度值显著低于对照组（P < 0.01）；感染Ad-Hes1的细胞系24 h和48 h的划痕愈合率显著低于对照组（P < 0.001）；感染Ad-Hes1的细胞系在Transwell小室培养48 h后迁移进入Transwell下室的细胞数量显著低于对照组（P < 0.001）；感染Ad-Hes1的细胞系在铺有Matrigel基质胶的Transwell小室培养48 h后侵袭进入Transwell下室的细胞数量显著低于对照组（P < 0.01）。
结论 Hes1有抑制Hep1-6细胞增殖、迁移与侵袭的作用。

Abstract:
Objective To investigate the effects of Hes1 gene on the proliferation, migration and invasion of hepatocellular carcinoma cell line Hep1-6.
Methods Cell proliferation, cell colony formation, migration and invasion were measured after Hes1 gene were overexpressed in Hep1-6 cells mediated by recombinant adenovirus.
Results The clone numbers of Hep1-6 cells infected with Ad-Hes1 were significantly less than the control group infected with Ad-GFP (P < 0.001). Six days after Ad-Hes1 infection, OD_450nm of Hep1-6 cells were significantly lower than that of control group (P < 0.01). The wound healing rates of Hep1-6 cells infected with Ad-Hes1 at 24h and 48h were significantly lower than those of the control group (P < 0.001). The number of Hep1-6 cells infected with Ad-Hes1 migrating into the lower chamber of Transwell after 48 h of culture was significantly lower than that of the control group (P < 0.001). The number of Hep1-6 cells infected with Ad-Hes1 invading into the lower chamber of Transwell after 48h of culture was significantly lower than that in the control group (P < 0.01).
Conclusion Hes1 could suppress the proliferation, migration and invasion of Hep1-6 cells.

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