奥沙利铂通过调控miRNA-7-5p/RAF-1促进胃癌SGC-7901细胞凋亡的研究

谷泽慧; 任立群; 李琪; 王亚帝; 谭琦; 娄小倩; 陈素贤

doi:10.3971/j.issn.1000-8578.2020.19.0695

奥沙利铂通过调控miRNA-7-5p/RAF-1促进胃癌SGC-7901细胞凋亡的研究

Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1

摘要

摘要:
目的探讨奥沙利铂如何调控MAPK通路，抑制胃癌细胞的增殖。
方法 NCBI检索文献，利用TargetScan、StarBase和miRBase数据库，进行GO分析与KEGG通路富集，找到相关miRNAs，预测靶基因。应用Real-time PCR、MTT、Hoechst33258、流式细胞术、细胞划痕实验、Western blot等方法分析人胃癌SGC-7901细胞的增殖、细胞周期、侵袭及蛋白表达情况。
结果胃癌细胞中miR-7-5p显著低表达，RAF1与miR-7-5p存在互靶关系。miR-7-5p mimics与奥沙利铂均可促进SGC-7901细胞的凋亡，提高G₁期细胞百分率（P < 0.05），降低侵袭、迁移速度。caspase3、caspase9蛋白表达升高，Bcl-2/Bax比值降低（P < 0.05）。
结论过表达miR-7-5p与奥沙利铂均可促进胃癌SGC-7901细胞的凋亡，提示奥沙利铂可能通过上调miRNA-7-5p促进SGC-7901细胞的凋亡，降低侵袭、迁移速度。

Abstract:
Objective To investigate how oxaliplatin regulates the MAPK pathway and inhibits the proliferation of gastric cancer cells.
Methods From NCBI, TargetScan, StarBase and miRBase databases were used to perform GO analysis and KEGG pathway enrichment in DAVID analysis, to find related miRNAs and predict their target genes. The proliferation, cell cycle, invasion and protein expression of SGC-7901 cells were analyzed by Real-time PCR, MTT, Hoechst33258, flow cytometry, scratch assay and Western blot, respectively.
Results miR-7-5p expression was significantly downregulated in gastric cancer cells. RAF1 and miR-7-5p were co-targeted. The apoptosis of SGC-7901 cells was promoted, the percentage of G₁ phase cells and the expression of caspase3 and caspase9 were increased, while the percentage of G₂ phase cells and the ratio of Bcl-2/Bax protein were decreased in miR-7-5p mimics and oxaliplatin (OXA) groups(P < 0.05).
Conclusion Both overexpression of miR-7-5p and oxaliplatin could promote the apoptosis of gastric cancer SGC-7901 cells, suggesting that oxaliplatin may promote the apoptosis of SGC-7901 cells and reduce the rates of invasion and migration by up-regulating miRNA-7-5p.

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