膀胱癌组织中色素上皮衍生因子与雄激素受体的表达及其相关性

蒋华; 卜强; 吴爱斌; 曾明辉; 秦锁炳; 夏东东

doi:10.3971/j.issn.1000-8578.2019.19.0132

膀胱癌组织中色素上皮衍生因子与雄激素受体的表达及其相关性

Expression and Correlation of Pigment Epithelium-derived Factor and Androgen Receptor in Bladder Cancer Tissues

摘要

摘要:
目的探讨膀胱癌组织中色素上皮衍生因子(PEDF)与雄激素受体(AR)的表达及其相关性。
方法采用免疫组织化学染色检测30例膀胱癌组织中PEDF和AR表达，采用Pearson相关性分析PEDF表达与AR之间的关系。Real-time PCR和Western blot检测在不同浓度的雄激素处理下，膀胱癌TCCSUP细胞株中PEDF和AR的表达水平以及慢病毒介导的AR干扰后，细胞株中PEDF的表达水平。
结果相关性分析表明PEDF表达与AR表达呈负相关(P=0.003)。雄激素处理后上调AR表达，抑制PEDF表达。同时，干扰AR的表达可以上调PEDF表达。
结论膀胱癌组织中PEDF的表达可能受到AR表达所抑制。

Abstract:
Objective To investigate the expression and correlation of pigment epithelium derived factor (PEDF) and androgen receptor (AR) in bladder cancer tissues.
Methods The expression of PEDF and AR in 30 cases of bladder cancer tissues were detected by immunohistochemical staining. The relation between PEDF expression and AR was analyzed by Pearson correlation. We detected the expression levels of PEDF and AR in bladder cancer TCCSUP cell line and the expression of PEDF after lentivirus-mediated AR interference by Real-time PCR and Western blot.
Results PEDF expression was negatively correlated with AR expression(P=0.003). Androgen could significantly upregulate AR expression and inhibit PEDF expression. At the same time, interfering AR expression could upregulate the expression of PEDF.
Conclusion The expression of PEDF may be inhibited by AR expression in bladder cancer tissues.

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