Abstract:
Objective To investigate the effects of microRNA-144 (miR-144) on the proliferation, migration, invasion and phosphoinositide-3 kinase (PI3K) pathway of pancreatic cancer SW1990 cells.
Methods The expression of miR-144 in human normal pancreatic epithelial cell line HPDE and pancreatic cancer cells SW1990 were detected by RT-qPCR. SW1990 cells were transfected with miR-144 negative control plasmid (negative transfection group) and miR-144 mimic plasmid (miR-144 overexpression group), and untreated cells were taken as blank control group. The expression level of miR-144 in each group was detected by RT-qPCR. CCK-8 assay was used to detect cell proliferation. Plate clone formation assay was used to detect colony formation. Transwell assay was used to detect cell migration and invasion. The expression levels of PI3K and phosphorylated protein (p-PI3K), serine-threonine protein kinase (Akt) and phosphorylated protein (p-Akt) proteins in PI3K pathway were detected by Western blot.
Results The expression level of miR-144 in SW1990 cells was significantly lower than that in HPDE cells (P < 0.05). The expression level of miR-144 in miR-144 overexpression group was significantly higher than those in blank control group and negative transfection group (P < 0.05). Cells proliferation rate, colony formation, the number of migration and invasion cells and the expression levels of p-PI3K and p-Akt proteins in the overexpression group were significantly lower than those in the blank control group and negative transfection group (all P < 0.05).
Conclusion miR-144 is lowly expressed in pancreatic cancer cells SW1990. The up-regulation of miR-144 expression could effectively inhibit the proliferation, migration and invasion of pancreatic cancer cells, which may be related to the inhibition of PI3K pathway.
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