口腔鳞癌MicroRNA-150表达及其与肿瘤相关巨噬细胞的相关性

彭宏峰; 王元杰; 田松波; 董伟; 梁永强; 董青; 马冬

doi:10.3971/j.issn.1000-8578.2018.18.0931

口腔鳞癌MicroRNA-150表达及其与肿瘤相关巨噬细胞的相关性

Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma

摘要

摘要:
目的探讨口腔鳞癌miRNA-150的表达及其与口腔癌相关巨噬细胞（tumor-associated macrophages, TAMs）的相关性和临床意义。
方法采用mRNA表达谱芯片分析3例口腔鳞癌和3例年龄、性别相匹配的正常口腔上皮黏膜组织，应用qRT-PCR和免疫双荧光染色方法检测45例口腔鳞癌组织及38例正常口腔黏膜组织中miR-150和CD68/IL-10蛋白的表达情况，并分析其与口腔鳞癌患者临床病理特征的关系。
结果与正常口腔组织比较，口腔鳞癌组织中miR-150和IL-10的表达均升高（均P < 0.05），并且CD68⁺IL-10⁺ TAM的浸润增加（P < 0.05）；口腔鳞癌组织中miR-150表达升高与患者病理分期、分化程度和淋巴结转移相关（均P < 0.05）。
结论 miR-150可能通过调控M2型巨噬细胞的表型转换发挥免疫抑制功能并参与口腔鳞癌的发生与发展。

Abstract:
Objective To investigate the expression of miRNA-150 and IL-10 in TAMs and its clinicopathological significance in OSCC.
Methods Different expression of miRNA in OSCC and control was screened by gene chip. The expression of miRNA-150 and CD68/IL-10 protein in oral tissues from 45 cases of OSCC and 38 cases of normal oral mucosa were detected by qRT-PCR and dual immunofluorescence staining. The relationship between miRNA-150 expression and clinical data was analyzed.
Results The expression of miRNA-150 and CD68/IL-10 in OSCC tissues was significantly higher than in normal tissues (P < 0.05). The infiltration of CD68⁺IL-10⁺ cells increased, and miRNA-150 was related to pathological staging, tumor differentiation and lymph node metastasis in patients with OSCC (P < 0.05).
Conclusion The expression of miRNA-150 and TAM-derived IL-10 in OSCC is increased, suggesting that miRNA-150 may promote phenotype transformation of M2 macrophages participating in immunosuppressive function and oral tumorigenesis.

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