荧光原位杂交检测乳腺癌HER2（++）扩增状态及其与临床病理的相关性

张明帅; 王胄; 蒋威华; 李涌涛; 甫拉提·吾瓦力汉; 欧江华

doi:10.3971/j.issn.1000-8578.2018.18.0179

荧光原位杂交检测乳腺癌HER2（++）扩增状态及其与临床病理的相关性

HER2 (++) Amplification in Breast Cancer Detected by Fluorescence in Situ Hybridization and Its Correlation with Clinicopathological Features

摘要

摘要:
目的使用荧光原位杂交（fluorescence in situ hybridization, FISH）检测HER2基因扩增情况，并探讨影响HER2基因扩增的因素及其与临床病理特征的关系。
方法收集新疆医科大学附属肿瘤医院2013年l月至2015年12月间IHC检测HER2（++）的乳腺癌病例325份，均采用IHC和FISH两种方法分别检测所有患者的石蜡标本HER2表达和扩增情况，并分析HER2扩增状态与患者各临床病理特征的关系。
结果全组患者经IHC检测HER2表达均为（++），FISH检测HER2扩增率为12.9%（42/325），对12项临床和病理指标进行单因素分析显示：HER2扩增状态与激素状态、肿瘤直径、P53显著相关（P < 0.05），而与ki67表达、组织学分级、肿瘤个数等因素均无关（P > 0.05）。
结论雌孕激素表达均阴性、肿瘤直径 > 2 cm、P53表达阳性是预测FISH检测IHC HER2（++）扩增的独立因素。

Abstract:
Objective To detect the amplification of HER2 gene by fluorescence in situ hybridization(FISH), and explore the correlation between HER2 gene amplification and clinicopathological characteristics.
Methods We collected 325 cases of breast cancer with HER2(++) in the Affiliated Tumor Hospital of Xinjiang Medical University from January 2013 to December 2015. All paraffin-embedded specimens were detected by IHC and FISH respectively for the expression of HER2 protein and amplification. And we analyzed the relationship between HER2 gene amplification and clinicopathological characteristics of patients.
Results HER2(++) of all groups was detected immunohistochemically. And the amplification rate of HER2 gene was 12.9%(42/325). Univariate analysis on 12 clinicopathological indexes showed that the amplification status of HER2 gene was significantly correlated with hormonal status, tumor diameter and P53, rather than ki67 expression, histological grade, tumor number or other factors.
Conclusion The negative expression of estrogen and progesterone, tumor diameter > 2 cm and positive P53 expression are the independent factors to predict IHC HER2(++) amplification by FISH.

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