核转录因子E47和LOXL2在食管鳞癌组织中的表达及其临床意义

马红; 张燕萍; 侯敏杰; 李金钰; 朱亚杰; 迪里胡马尔.艾海提; 郭琼; 马遇庆

doi:10.3971/j.issn.1000-8578.2018.18.0137

核转录因子E47和LOXL2在食管鳞癌组织中的表达及其临床意义

Significance of E47 and LOXL2 Expressions in Esophageal Squamous Cell Carcinoma Tissues

摘要

摘要:
目的探讨核转录因子E47和赖氨酰氧化酶样蛋白2（LOXL2）在食管鳞癌组织中的表达及其与临床病理特征的关系。
方法采用实时荧光定量PCR和免疫组织化学方法检测E47和LOXL2在食管鳞癌组织及癌旁正常组织中mRNA和蛋白的表达情况，并分析其与临床病理特征的关系。
结果 E47和LOXL2 mRNA在食管鳞癌组织中的表达量明显高于癌旁正常组织（均P < 0.05）。E47和LOXL2蛋白在食管鳞癌组织中的阳性表达率明显高于癌旁正常组织（均P < 0.05）；E47蛋白表达与浸润深度密切相关，LOXL2蛋白表达与分化程度、淋巴结转移密切相关。食管鳞癌组织中E47与LOXL2蛋白表达呈正相关关系（r=0.582, P < 0.05）。
结论 E47和LOXL2 mRNA和蛋白在食管鳞癌组织中高表达，并与临床病理特征相关。E47和LOXL2高表达可能参与食管鳞癌的侵袭与转移，可作为判断食管癌预后的分子标志物。

Abstract:
Objective To investigate the relationship of E47, lysyl oxidase like2 protein(LOXL2) expressions with clinicopathologic parameters of esophageal squamous cell carcinoma(ESCC) patients.
Methods The mRNA and protein levels of E47 and LOXL2 in ESCC and adjacent tissues were detected by qRT-PCR and immunohistochemistry method, and their correlation with the clinicopathologic parameters were analyzed.
Results The expressions of E47 and LOXL2 mRNA in ESCC tissues were significantly higher than those in adjacent normal tissues (all P < 0.05). The positive expressions of E47 and LOXL2 proteins in ESCC were higher than those in adjacent tissues (all P < 0.05). The expression of E47 protein was closely correlated with invasion depth. The expression of LOXL2 protein was both significantly correlated with the level of histological differentiation and lymphatic metastasis (P < 0.05). E47 protein expression was positively correlated with LOXL2 expression (r=0.582, P < 0.05).
Conclusion The mRNA and protein levels of E47 and LOXL-2 are highly expressed in ESCC tissues, and significantly correlated with clinicopathologic characteristics. High expressions of E47 and LOXL2 may promote the invasion and metastasis of ESCC, and they are expected to be the potential biomarkers for the prognosis.

HTML全文

参考文献(19)

施引文献

资源附件(0)