β1, 3-乙酰葡萄糖胺转移酶3在非小细胞肺癌中的表达及其临床意义

李斌峰; 向正凯; 熊飞; 王华斌; 严宝国

doi:10.3971/j.issn.1000-8578.2018.17.1638

β1, 3-乙酰葡萄糖胺转移酶3在非小细胞肺癌中的表达及其临床意义

Expression of β1, 3-N-acetylglucosaminyltransferase-3 in Non-small Cell Lung Cancer and Its Clinical Significance

摘要

摘要:
目的探讨β1, 3-乙酰葡萄糖胺转移酶3（β1, 3-N-acetylglucosaminyltransferase-3, B3GNT3）在非小细胞肺癌（non-small cell lung cancer, NSCLC）组织中的表达及其临床意义。
方法通过在线TCGA数据库分析B3GNT3基因的表达；Western blot法及免疫组织化学法检测60例NSCLC组织及22例正常肺组织中B3GNT3蛋白的表达情况，分析B3GNT3蛋白表达与NSCLC患者临床病理特征及预后之间的关系。
结果 NSCLC患者组织中B3GNT3基因和蛋白表达明显高于正常肺组织，差异有统计学意义（P < 0.01）。B3GNT3蛋白表达与NSCLC患者的年龄、性别、吸烟情况、种族、TNM分期均无明显关系（P > 0.05）。B3GNT3高表达组患者生存期明显短于B3GNT3低表达组患者（P < 0.05）。
结论 B3GNT3蛋白在NSCLC组织中高表达，且其表达高低与NSCLC患者预后有关。

Abstract:
Objective To investigate the expression of β1, 3-N-acetylglucosaminyltransferase-3 gene (B3GNT3) in non-small cell lung cancer (NSCLC) tissues and its clinical significance.
Methods The expression of B3GNT3 gene was analyzed by online TCGA database. The expressions of B3GNT3 protein in NSCLC tissues (n=60) and normal lung tissues (n=22) were detected by Western blot and immunohistochemical method. The relationship between the expression of B3GNT3 protein and the clinicopathological features of NSCLC patients was analyzed.
Results The B3GNT3 expression in NSCLC tissues was significantly higher than that in normal lung tissues (P < 0.01). The positive expression of B3GNT3 protein was not associated with age, gender, smoking status, race or TNM stage (P > 0.05). The overall survival time of NSCLC patients with high expression of B3GNT3 protein was shorter than that with low expression of B3GNT3 protein (P < 0.05).
Conclusion The expression of B3GNT3 protein is highly expressed in NSCLC tissues, and the expression level of B3GNT3 protein is associated with the prognosis of NSCLC patients.

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