SNAI2基因对低转移潜能乳腺癌细胞恶性特征的影响及机制

周园红; 罗幼珍; 刘强; 刘晓雯

doi:10.3971/j.issn.1000-8578.2018.17.1408

SNAI2基因对低转移潜能乳腺癌细胞恶性特征的影响及机制

Effects and Regulatory Mechanisms of SNAI2 on Malignant Hallmarks of Non-Invasive Breast Tumor Cells

摘要

摘要:
目的探讨SNAI2基因对低转移潜能乳腺癌MCF-7和T-47D细胞恶性特征的影响及潜在机制。
方法（1）采用慢病毒载体筛选SNAI2过表达的MCF-7和T-47D细胞；（2）采用MTT法检测细胞增殖能力，划痕实验检测细胞迁移能力，Hoechst 33342检查细胞抗凋亡能力；（3）Real-time PCR法检测细胞基因表达水平。
结果（1）经荧光显微镜观察证实SNAI2已成功转染入MCF-7和T-47D细胞中；（2）SNAI2的高表达能增强MCF-7和T-47D细胞的恶性特征，包括增殖、侵袭迁移及抗凋亡能力（P < 0.01）；（3）SNAI2的高表达可促进MCF-7和T-47D细胞中Cyclin D1、MMP9和VIM的表达水平上调，E-CDH的表达水平下调（P < 0.01）。
结论 SNAI2可通过促进低转移潜能乳腺癌细胞发生上皮细胞-间充质转化（EMT）获得更高的转移潜能，为进一步探索SNAI2在乳腺癌的基因靶向治疗提供新的方法和理论支撑。

Abstract:
Objective To explore the effects and related mechanisms of SNAI2 on malignant hallmarks of non-invasive breast tumor MCF-7 and T-47D cells.
Methods (1) The lentiviral vector was used to screen MCF-7 and T-47D cells with SNAI2 overexpression; (2) The proliferation, migration and anti-apoptosis abilities of tumor cells were detected by MTT assay, wound healing and Hoechst 33342 assay, respectively; (3) The gene expression was tested by real-time PCR.
Results (1) The GV367-SNAI2 was successfully transfected into MCF-7 and T-47D cells; (2)The SNAI2 could enhance malignant hallmarks of non-invasive breast tumor cells, including the proliferation, migration and anti-apoptosis abilities(P < 0.01); (3) The over-expression of SNAI2 could up-regulate the expression of Cyclin D1, MMP9, VIM and down-regulate the expression of E-CDH in MCF-7 and T-47D cells(P < 0.01).
Conclusion SNAI2 could promote higher metastasis potential of non-invasive breast tumor cells through EMT, which will provide the experimental foundation for further exploring new targeted therapeutic method.

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