SFRP1基因甲基化及其蛋白在肺腺癌中的表达及临床意义

李小杰; 殷桂林; 董永强; 朱水波; 张晓明; 纪涛; 徐家行

doi:10.3971/j.issn.1000-8578.2017.05.002

SFRP1基因甲基化及其蛋白在肺腺癌中的表达及临床意义

Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance

摘要

摘要:
目的探讨SFRP1基因甲基化及其蛋白在肺腺癌组织中的表达。
方法收集手术切除的肺腺癌标本60例和癌旁组织30例，采用甲基化特异性PCR法、实时荧光定量PCR法和免疫组织化学染色法分别检测SFPR1基因甲基化、mRNA及蛋白的表达情况。
结果 SFRP1基因甲基化率在肺腺癌中（58.3%）表达明显高于癌旁组织（20.0%）（P=0.001）。SFRP1 mRNA在肺腺癌（0.847±0.297）中的表达明显低于癌旁组织（2.019±0.445）（P < 0.01）。SFRP1蛋白在肺腺癌中的阳性表达率为38.3%（23/60），明显低于癌旁组织中的表达率83.3%（25/30）（P < 0.001）；SFRP1蛋白表达与肺腺癌组织分化程度、临床分期及淋巴结转移情况有关（均P < 0.05）。SFRP1基因甲基化与其蛋白表达缺失存在相关性（r=-0.585, P < 0.001）。
结论 SFRP1蛋白的表达降低可能是由于SFRP1基因甲基化所致，SFRP1基因甲基化及其蛋白表达与肺腺癌组织分化程度及淋巴结转移情况密切相关。

Abstract:
Objective To investigate the expression of SFRP1 gene methylation and SFRP1 protein in lung adenocarcinoma tissues.
Methods There were 60 lung adenocarcinoma patients who underwent a completely surgical excision from May 2015 to May 2016. The expression of SFRP1 gene methylation, SFRP1 mRNA and SFRP1 protein in lung adenocarcinoma tissues and adjacent tissues were detected respectively by methylation specific PCR, real-time PCR and immunohistochemistry.
Results SFRP1 gene methylation rate was higher in tumor tissues than that in adjacent tissues (58.3% vs. 20.0%, P=0.001). The relative expression of SFRP1 mRNA in tumor tissues and adjacent tissues were (0.847±0.297) and (2.019±0.445) (P < 0.01). The positive expression rate of SFRP1 protein in tumor tissues was significantly lower than that in adjacent tissues (38.3%(23/60) vs. 83.3%(25/30), (P < 0.001)). SFRP1 protein expression was correlated with differentiation degree, clinical stage and lymph node metastasis (P < 0.05). SFRP1 gene methylation was closely correlated with SFRP1 protein expression deficiency in lung adenocarcinoma tissues (r=-0.585, P < 0.001).
Conclusion The expression of SFRP1 gene methylation and protein are associated with the degree of differentiation and lymph node metastasis. The decrease of SFRP1 protein expression may be due to SFRP1 gene methylation.

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