肝细胞肝癌间质微环境与TLR3表达的相关性及其对预后的影响

张崇辉; 陈莉

doi:10.3971/j.issn.1000-8578.2017.04.005

肝细胞肝癌间质微环境与TLR3表达的相关性及其对预后的影响

张崇辉,
陈莉

Correlation Between Stromal Microenvironment and TLR3 Expression, Prognosis Respectively in Hepatocellular Carcinoma

摘要

摘要:
目的分析肝细胞肝癌（hepatocellular carcinoma, HCC）间质微环境与Toll样受体3（Toll-like receptor 3, TLR3）表达的相关性及其对预后的影响。
方法利用已有专利技术制备人体HCC组织芯片，通过免疫组织化学检测HCC间质中各项指标及HCC细胞中TLR3表达，各因素相关分析和Kaplan-meier生存分析上述指标与TLR3的关系，及其与HCC预后的关系。
结果 HCC间质微环境中浸润的T细胞（P=0.002）、Kupffer细胞（P=0.049）、自然杀伤（natural killer, NK）细胞（P=0.000）和树突状细胞（dendritic cells, DCs）（P=0.027）与TLR3表达呈正相关，而肥大细胞（P=0.000）、癌相关肌纤维母细胞（carcinoma-associated fibroblasts, CAFs）（P=0.000）和微血管密度（microvessel density, MVD）（P=0.000）与TLR3表达呈负相关；Kupffer细胞（P=0.013）和NK细胞（P=0.001）与预后呈正相关，而肥大细胞（P=0.008）、CAFs（P=0.000）和MVD（P=0.089）与预后呈负相关。
结论间质微环境在HCC的演进过程中起着重要作用。

Abstract:
Objective To analyse the correlation between the stromal microenvironment and the Toll-like receptor 3(TLR3) expression, the prognosis respectively in hepatocellular carcinoma (HCC).
Methods The tissue microarrays of human HCC were prepared with self-owned patent technology. The expression of various indexes in HCC stroma and TLR3 in HCC cells were examined with immunohistochemistry. The correlation between the indexes with TLR3 and theirs relationship with the prognosis of HCC were analyzed by multi-factor correlation analysis as well as survival analysis of Kaplan-Meier.
Results T cells, Kupffer cells, natural killer (NK) cells and dendritic cells (DCs) had positive correlation with the TLR3 expression in HCC. Mast cells, carcinoma-associated fibroblasts (CAFs) and microvessel density (MVD) had negative correlation with the TLR3 expression in HCC. Kupffer cells and NK cells were positively related with the prognosis of HCC. Mast cells, CAFs and MVD were negatively related with the prognosis of HCC.
Conclusion The stromal microenvironment of HCC plays an important role in HCC evolution.

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