Abstract:
Objective To explore effect of cisplatin combined with Buzhong Yiqi decoction on survivin expression in transplantation tumor of nude mice with A549/DDP cells.
Methods Thirty BALB/c-nu mice were randomly divided into five groups: A group (tumor control group),B group (cisplatin group),C group (cisplatin with low dose of Buzhong Yiqi decoction),D group (cisplatin with medium dose of Buzhong Yiqi decoction),E group (cisplatin with high dose of Buzhong Yiqi decoction). DDP or DDP and Buzhong Yiqi decoction were treated from 9 to 33 day,lastly the mice were executed. The volume and weight of transplantation tumors were measured; tumor inhibitory rate was calculated. Real-time PCR was used to determine the expression of survivin mRNA; Western blot was adopted to detect the expression of survivin protein.
Results The volume and weight of transplantation tumors in C,D and E groups were significantly smaller than those in A and B groups. Tumor inhibitory rates were increased with the increasing dose of Buzhong Yiqi decoction. Compared with A group,the survivin gene and protein levels of transplantation tumors in B and C groups were higher,however,the results were lower in D and E groups. The survivin gene and protein expressions of transplantation tumors in C,D and E groups were significantly down-regulated,compared with B group.
Conclusion Cisplatin combined with Buzhong Yiqi decoction could improve cisplatin resistance of transplantation tumor of nude mice with A549/DDP cells through down-regulating the gene and protein expression of survivin.
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