Abstract:
Objective To explore the effect of the 5th subunit of β-HCG, chorionic gonadotropin beta polypeptide 5 (CGB5), on human epithelial ovarian cancer cell line OVCAR-3 in vasculogenic mimicry (VM) formation.
Methods The CGB5 overexpressed vector, blank vector, CGB5 siRNA and scramble siRNA were transfected into OVCAR-3 cells. The OVCAR-3 cells without treatment were taken as blank control. The human choriocarcinoma cells BeWo with CGB5 high expression was used as a positive control. These cells were injected subcutaneously into the nude mice. When the tumors grew to an average size of 1-2 cm3, the mice were sacrificed by cervical decapitation. The HE staining, CD34-PAS dual staining and transmission electron microscopy were used to observe the morphology characteristic of VM in xenografts.
Results Compared with the control groups, VM channels in xenografts were increased significantly in CGB5- overexpressed OVCAR-3 cells group and reduced significantly in CGB5-knockdown OVCAR-3 cells group.
Conclusion CGB5 may promote the formation of VM, it may provide a new way for ovarian cancer therapy.
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