Abstract: Objective To investigate the effect of siRNA silencing BRG1 gene on glioma cell proliferation and its molecular mechanism. Methods Chemically synthesized small interfering RNA targeting BRG1 was transfected into human glioma cell lines U251 and U87 by siLentFect Lipid Reagent. The proliferation of both glioma cells after BRG1 interference was measured by CCK-8 assay. The cell cycles of glioma cells were detected by flow cytometry.The expression levels of BRG1, cyclin family and CDK inhibitor proteins were detected by western blot. Results BRG1 interference could drastically decrease the ability of cell proliferation in both glioma cells. The G1 population in both glioma cells treated by BRG1 siRNA was increased signifi cantly than that in the cells treated by control siRNA. The Western blot results showed that BRG1 knock-down inhibited expression of both cyclin D1 and cyclin B1, but the expression of p21 and p27. Conclusion BRG1 siRNA could effectively decrease the expression of BRG1 in glioma cell and arrest cell cycle at G1 phase and signifi cantly inhibit the cell proliferation.