Abstract: Objective To study the effect of drug-containing serum of Hui medicine AiKang Recipe on proliferation and apoptosis of Lewis lung cancer cells (LLC) in vitro and its anti-tumor mechanism. Methods A total of 80 male SD rats were divided into 8 groups, low, medium, high dose of medicine AiKang Recipe(AKL\AKM\AKH) groups, cyclophosphamide group(CTX), low, medium, high dose of AiKang Recipe combined with CTX(AKL+CTX\AKM+CTX\AKH+CTX) groups and normal saline(NS) group. The drug-containing serum was prepared by the serum pharmacology method to culture LLC in vitro. The proliferation of LLC was measured by methyl thiazolyl tetrazolium (MTT) method. The cell apoptosis was measured by fl ow cytometry (FCM) and Annexin V-FITC/PI. Results Drug-containing serum of Hui medicine AiKang Recipe had a remarkable growth-inhibition on LLC in vitro in time- and concentrationdependent manners. The inhibition rate(IR) was 26.92% after 20% drug-containing serum in AKH group for 72 h. Inhibition rate was up to 54.71% in high dose of AiKang Recipe combined with CTX. Compared with the control group, each group using drug-containing serum could induce apoptosis of LLC in timeand concentration-dependent manners. AiKang Recipe combined with CTX containing drug serum on LLC for 72h had the strongest effect, ie. apoptosis rate declined while the necrosis increased. Conclusion Hui medicine AiKang Recipe alone or combined with CTX inhibited proliferation and induce apoptosis of LLC.