Abstract: Objective To develop a rapid,high-throughput screening method of suspension array technique to simultaneously detect matrix metallo protenases 9(MMP9),heparanase(Hpa),and cathepsin L(CL) in serum,and to explore their clinical diagnosis value for ovarian cancer.Methods The suspended arrays of MMP9,Hpa and CL were prepared by adopting the fluorescent microspheres coated with antibody.Suspension array technology and enzyme-linked immunosorbent assay were performed to detect serum concentrations of MMP9,Hpa and CL in 83 patients with ovarian cancer,50 cases of benign ovarian tumor and 55 cases of healthy women.The sensitivity of two methods was analyzed.Results The dynamic range of suspension array was 7~2 056 u/ml,coefficient of variation was 3.39%~6.15%.The corresponding linear range of enzyme-linked immunosorbent assay was 20~1 024 u/ml,coefficient of variation range was 6.45%~11.82%.The sensitivity and specificity of MMP9,Hpa and CL combined detection by suspension array were 90.4% and 92.0% respectively in diagnosis of ovarian cancer. Similarly the sensitivity and specificity were 91.1%,92.6% in diagnosis of ovarian cancer before surgery. Conclusion Suspension array of MMP9,Hpa and CL has higher sensitivity and wider dynamic range than ELISA method in detecting serum protein,and is a high-throughput,multi-factor quantitative rapid detection for ovarian cancer.