Abstract: Objective To discuss the effects and mechanism of MAGEA10 expression in human lung cancer A549 cells influenced by Viili polysaccharides. Methods MTT was used to study the survival impact of non small cell lung cancer (NSCLC) A549 with viili polysaccharides in different concentration at 24 h,48 h and 72 h. qRT-PCR was used to detect the relative expression changes of MAGEA10 mRNA with Viili polysaccharides in different concentration of 10 mg/L, 25 mg/L and 50 mg/L compared with those of the normal A549 cells. And Western blot was adopted to detect the antigen expression of MAGEA10 after stimulation of A549 with Viili polysaccharides in different concentration of 10 mg/L, 25 mg/L and 50 mg/L. Through the expression of MAGEA10 mRNA and antigen, we discussed the effects and possible mechanism of MAGEA10 expression made by Viili polysaccharides. Results (1) Viili polysaccharides inhibited cell proliferation of A549 and its concentration was negatively related to cell survival within 0 mg/L-50 mg/L and the result was most remarkable at 48 h. (2) Compared with the blank group, the relative expression of MAGEA10 mRNA elevated at the concentration of 50 mg/L. (3) Compared with the blank group, the protein expression of MAGEA10 elevated,at the concentration of 50 mg/L. Conclusion Viili inhibited NSCLC A549 cell proliferation, but the increase of the gene transcription and translation levels of MAGEA10 in A549 cell needs further exploration for those two parts might be interdependent. This study aims to indicate that viili is possible to promote non specific immunity, and increase possibility of cancer cell recognition by CTLs, and possibly initiate apoptotic process of cancer cells if same epitope of MAGEA10 and corresponding TCR encounters.