Abstract: Objective To investigate the clinical manifestations,treatment and prognosis of granulocytic sarcoma (GS). Methods A retrospective analysis regarding the clinical characteristics and treatment was performed in 28 GS patients.In addition,there was a clinical follow-up.Meanwhile, survival analysis was done using Kaplan-Meier,so did the significant difference test using Log rank. Results There were 18 males and 10 females,with a median age of 40 years.The predilection sites included lymph nodes,subcutaneous tissue,bone,breast and so on The total two-year survival rate was 21.4%.Fourteen patients were misdiagnosed when initially diagnosed,significant difference was identified between their survival rate and those were not (P=0.018).There was no significant difference in survival rate between leukemic and non-leukemic GS patients(P=0.093).The median survival time of patients with normal karyotypes was significantly longer than those with abnormal ones (22 months vs.8 months).(P=0.049). And survival rate difference was statistically significant among different theraputic groups (P=0.031).The median survival time of patients received bone marrow stem cells transplantion was significantly longer than the conventional therapy group and high-intensity chemotherapy group (36 months vs.17,22 months,respectively). Conclusion GS is frequently misdiagnosed due to the diverse clinical manifestations.Besides,it relapses easily and tends to have poor prognosis,especially in those with abnormal karyotype.Therefore,chemotherapy should be timely conducted after confirmed diagnosis.Our outcomes also indicate that early intensive chemotherapy may delay relapsing and improving long-term survival rate.Moreover,hematopoietic stem cell transplantation can significantly prolong the survival time and even improve survival quality.