塞来昔布对NB4细胞增殖、凋亡及VEGF表达的影响

doi:10.3971/j.issn.1000-8578.2013.02.006

塞来昔布对NB4细胞增殖、凋亡及VEGF表达的影响

Effects of Celecoxib on Proliferation,Apoptosis and VEGF Expression in NB4 Cells

摘要

摘要: 目的探讨塞来昔布对急性早幼粒细胞白血病细胞的抑制作用及可能机制。方法不同浓度塞来昔布处理急性早幼粒细胞白血病细胞株NB4，MTT法检测塞来昔布作用后细胞的增殖情况。采用流式细胞术测定NB4细胞周期分布及凋亡；Western blot检测细胞凋亡相关蛋白Bcl-2表达以及RT-PCR检测塞来昔布作用对VEGF、HIF-1α 在mRNA水平的表达。结果MTT显示塞来昔布对NB4细胞生长有明显抑制作用。20~80 μmol/L塞来昔布作用24 h，NB4细胞G₀/G₁期比例明显升高，而S期细胞比例下降(P＜0.05)；塞来昔布处理组细胞凋亡率明显高于对照组，Western blot显示Bcl-2蛋白表达下调(P＜0.05)；RT-PCR显示塞来昔布可抑制VEGF及HIF-1α的mRNA表达。结论塞来昔布在体外可抑制急性早幼粒细胞白血病细胞增殖并诱导凋亡。

Abstract: ObjectiveTo explore effects of celecoxib on proliferation,apoptosis and VEGF expression in acute promyelocytic leukemia cell (NB4). Methods After acute promyelocytic leukemia cell line NB4 was treated by different concentrations of celecoxib,cell proliferation was detected by MTT assay.NB4 cell cycle distribution and apoptosis were measured by flow cytometry.Apoptosis related protein,the Bcl-2 expression and VEGF,HIF-1α mRNA levels were detected by Western blot and RT-PCR,respectively. Results The proliferation of NB4 cells was significantly inhibited by celecoxib.Celecoxib could increase the percentage of G₀/G₁cells and decrease S phase cells.The rate of apoptosis cell was increased in NB4 cells with 20-80 μmol/L celecoxib treatment for 24h.Celecoxib significantly induced apoptosis,and down-regulated Bcl-2 protein (P＜0.05).VEGF and HIF-1α mRNA levels were inhibited in NB4 cells after celecoxib treatment. Conclusion Celecoxib could inhibit cell proliferation,arrest cycles and induce apoptosis,through down-regulation of VEGF and HIF-1α in acute promyelocytic leukemia cells in vitro.

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