hTERTC27过表达对鼻咽癌C666-1细胞株增殖和凋亡的影响

doi:10.3971/j.issn.1000-8578.2013.01.008

hTERTC27过表达对鼻咽癌C666-1细胞株增殖和凋亡的影响

Effect of hTERTC27 Overexpression on Prolifteration and Apoptosis of Nasopharyngeal Carcinoma C666-1 Cell Line

摘要

摘要: 目的研究外源表达hTERTC27对鼻咽癌C666-1细胞增殖和凋亡的影响。方法采用脂质体将pcDNA3-hTERTC27转染至C666-1细胞株中。采用Western blot法鉴定hTERTC27和Bcl-2在细胞中的表达。采用MTT比色法和细胞克隆形成实验观察其对细胞增殖的影响。采用TUNEL实验观察其对细胞凋亡的影响。结果脂质体转染24h后，C666-1细胞株外源性hTERTC27出现高表达。过量表达hTERTC27的C666细胞增殖能力明显减缓（P<0.01），克隆形成率降低（P<0.01），TUNEL阳性细胞率增加（P<0.01），Bcl-2表达量降低（P<0.01）。结论 hTERTC27可以抑制C666-1细胞增殖、促进凋亡，可能与下调Bcl-2蛋白的表达有关。

Abstract: Objective To explore the effect of hTERTC27 overexpression on the proliferation and apoptosis of nasopharyngeal carcinoma C666-1 cell line. Methods pcDNA3-hTERTC27 was transfected into nasopharyngeal carcinoma C666-1 cells with lipofectamine.The expression of hTERTC27 and Bcl-2 were detected by Western blot.The proliferation of cells was detected by MTT assay and colony formation assay.The apoptosis of cells was detected by TUNEL assay. Results C666-1 cells expressed high level of hTERTC27 after 24h transfection.Over expression of hTERTC27 obviously suppressed the cell proliferation（P<0.01）,downregulated the colony formation rate（P<0.01）,increased the TUNEL positive cells （P<0.01），and downregulated the expression level of Bcl-2 protein（P<0.01）. Conclusion Overexpression of hTERTC27 can effectively inhibit proliferation and promote apoptosis of nasopharyngeal carcinoma cells,which is possibly due to the down regulation of Bcl-2 protein.

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