人参皂苷Rg3对小鼠Lewis肺癌生长和转移的抑制作用及其与肿瘤相关巨噬细胞的关系
Inhibitory Effect of Ginsenoside Rg3 on Growth，Metastasis and Expression of TAMs in Mice Lung Adenocarcinoma
-
摘要: 目的 研究人参皂苷Rg3(ginsenoside Rg3,Rg3)对肺腺癌小鼠异体移植瘤生长和转移的抑制作用及其相关机制。方法构建高转移肺腺癌小鼠移植瘤模型，将24只接种高转移性Lewis肺癌的C57BL/6J小鼠随机分成3组：对照组、顺铂(Cisplatin,DDP)组和Rg3组。给予相应药物干预，于接种后24日处死各组小鼠，剥离皮下肿瘤，取出双肺，计算肺转移发生情况，免疫组织化学检测皮下移植瘤中肿瘤相关巨噬细胞（tumor-associated macrophages，TAMs）和微血管密度（microvessel density，MVD）的表达并定量分析。结果DDP组、Rg3组抑瘤率分别为39.20%、54.86%，与对照组比较，瘤重差异有统计学意义（P<0.01），肺表面结节转移抑制率分别为30.25%，58.57%，与对照组比较差异有统计学意义（P<0.01），对照组、DDP组和Rg3组TAMs分别为(47.21±12.06)、(45.67±11.90)、(16.11±6.32)，MVD分别为(24.57±4.59)、(23.52±4.74)、(14.17±2.43)。结论Rg3对肺腺癌移植瘤的生长和转移具有抑制作用，其机制可能与其抑制肿瘤内TAMs在肿瘤基质中浸润及抑制微血管生成有关。Abstract: ObjectiveTo study the effects of suppressing lung adenocarcinoma growth and metastasis of ginsenoside Rg3 and its mechanism. Methods Twenty-four C57BL/6J mice bearing high metastatic Lewis cells were randomized into three groups: control group，DDP group and Rg3 group. Each group was treated with the appropriate intervention. All the mice were sacrificed by tumor inoculation in 24 days.Lung and subcutaneous tumors were peeled off for histological examination.The metastatic tumor foci on lung surface in mice were observed.Lung metastatic occurring rate and metastatic foci inhibitory rate were counted.CD68 and MVD were determined by immunohistochemistry method with image analysis system. ResultsThe growth inhibition rate in the DDP group and Rg3 group was 39.20% and 54.86%,respectively. Compared with the control group，the weight of tumor in the DDP group and Rg3 group showed a significant difference (P<0.01). The metastatic inhibitory rate in the DDP group and Rg3 group was 30.25% and 58.57%，respectively，which showed significan difference with the control group. great synergetic effect in decreaseing metastatic foci on lung surface. with.The expression of TAMs in the control group，cisplatin group and Rg3 group was (47.21±12.06)，(45.67 ±11.90) and (16.11±6.32)，respectively; and the expression of MVD were (24.57±4.59)，(23.52±4.74) and (14.17± 2.43)，respectively. Conclusion Rg3 can produce a synergetic inhibitory activity against the growth and lung metastasis of lung adenocarcinoma. The fact that Rg3 inhibited growth and lung metastasis of lung adenocarcinoma may be associated with its effect of suppressing the expression of TAMs and MVD in tumor tissue.
下载:
