Abstract: Objective To explore the function of PTTG and MMP-9 protein expression and evaluate their relationship with clinical stage of esophageal carcinoma. Methods Immunohistochemistry SP was used to check the protein expression of PTTG and MMP -9 in biopsy tissue specimen of 84 cases esophageal carcinoma,and the relationship between protein expression level and clinical stage grades was analyzed. Results PTTG and MMP-9 protein were high expression and with positive correlation in esophageal carcinoma.Positive expression rate were higher in late clinical stage than that in early stage in esophageal carcinoma. Conclusion PTTG and MMP-9 are high expression,and the two sorts of protein expression have a positive correlation with clinical stage of esophageal carcinoma.It suggests that PTTG and MMP -9 genes play an important role in regulating occurre,development,invasion and metastasis of esophageal carcinoma.