Abstract: Objective To study the expressions of cancer/testis antigens NY-ESO-1 and MAGE-A1 in breast carcinoma tissue. Methods According to pathological grade and pathologic type，90 breast cancer patients were studied,20 cases of normal breast gland as a control group.Immunohistochemical SP method was performed to detect the expression of antigens NY-ESO-1 and MAGE-A1 and the positive rate was measure by Tanaka-improved quantitative scoring method. Results There was no expression in normal breast tissue.The positive expression rate of NY-ESO-1 and MAGE-A1 was 37.78% and 23.33% respectively in breast cancer tissue,and had no obvious relevance to clinical pathological features (P>0.05) ConclusionNY-ESO-1 and MAGE-A1 were expressed with high specificity in breast carcinoma tissue,have slightly clinical significance in diagnosis and immunotherapy of breast carcinoma.