Abstract: Objective To explore the relationship between of the promoter hepomethylation of uPA gene and invasion and metastasis of nasopharyngeal cancer. Methods Methylation-specific polymerase chain reaction (PCR) and reverse transcription PCR techniques were used to determine the promoter methylation status and mRNA expression of uPA gene in nasopharyngeal cancer and chronic inflammation tissue in pharynxnasalis. Results Hepomethylation of uPA gene promoter was found in 51 of 58 nasopharyngeal cancer samples.Hepermethylation of uPA gene promoter was found in the other seven nasopharyngeal cancer tissues.Hepermethylation of uPA gene promoter was found in all chronic inflammation tissue of pharynx nasalis.Over-expression of uPA mRNA was found in nasopharyngeal carcinoma tissues which promoter hepomethylation.Weak or no expression of uPA mRNA was found in nasopharyngeal carcinoma which promoter hepermethylation cance and chronic inflammation tissue in pharynx nasalis. Conclusion Over-expression of uPA gene is relate to promoter hepomethylation in nasopharyngeal cancer tissues.Promoter hepomethylation of uPA gene possiblely concerned with invasion and metastasis of nasopharyngeal cancer.