热休克蛋白A5基因多态性与乙肝病毒侵袭的关系

Association between Polymorphism of Heat-shock Protein A5 and HBV Invasion

摘要: 目的探讨热休克蛋白A5基因（HSPA5）与乙型肝炎病毒（HBV）侵袭的关系。方法对210例HBV携带者和194例性别年龄匹配的健康者标本的PCR产物进行基因测序分型。酶联免疫吸附试验（ELISA）或临床化学方法检测HBV感染的血清学标志物。结果病例组的等位基因和基因型分布与对照组相比差异无统计学意义。此外，HBV感染的个体中，ALT/HBeAg和HBV-DNA均与等位基因或基因型的变异无相关性。结论HSPA5基因的-86 bp T/A多态性与HBV侵袭的临床风险和急性恶化没有相关性。

Abstract: Objective To explore the association between heat-shock protein A5(HSPA5) and hepatitis B virus(HBV) invasion. Methods DNA was genotyped for the single-nucleotide polymorphism(SNP) by polymerase chain reaction and sequencing in 210 unrelated HBV carriers and a total of 194 gender- and age-matched healthy controls.Serological markers for HBV infection were determined by enzyme-linked immunosorbent assay(ELISA) kits or clinical chemistry testing. Results The distributions of allele and genotype in cases were not significantly different from that in controls.In addition，neither ALT/HBeAg nor HBV-DNA was associated with the allele/genotype prevalence in HBV infected individuals. Conclusion -86 bp T/A polymorphism in HSPA5 gene is not related to the clinical risk and acute exacerbation of HBV invasion.