Abstract: Objective To explore the relationship between polymorphism of epidermal growth factor (EGF) 61A/G and the risk of hepatocellular carcinoma(HCC). MethodsElectronic searches of PubMed，EMBASE and CNKI were systematically conducted to select studies.Case-control studies containing available genotype frequencies of EGF 61A/G were chose.Odds ratio (OR) with 95% confidence interval (CI) was used to assess the strength of this association. Results Six case-control studies including 1 304 cases and 2 613 controls were performed.Meta-analysis showed significant effect of EGF 61A/G on HCC risk and protection (G/G vs.G/A+A/A：OR=1.34，95%CI=1.16~1.54；A/A vs.G/G+G/A：OR=0.66，95% CI=0.53~0.83). Conclusion The EGF 61G allele is a risk factor of HCC;however，the EGF 61A allele is a protective factor of HCC.