Abstract: Objective To investigate the expression and their clinical significance of VEGF，MVD and LN in normal colorectal mucosa，colorectal adenomas and colorectal carcinoma. Methods Expression of VEGF and LN in colorectal cancer，colorectal adenomas and colorectal normal tissues were detected by immunohistochemical staining，and the relationships were analyzed with the expression of VEGF，MVD and LN and micrometastasis in colorectal carcinoma. Results In the process that normal colorectal gradually developing into colorectal cancer，expression of VEGF，MVD and basement membrane obvious defect rate gradually increased(LN expression gradually weakened），the positive expression rate and MVD was related with depth of invasion of colorectal cancer，lymph node metastasis，Dukes staging (P<0.05)，but not with the degree of differentiation，patients′ gender，age，tumor location，histological type independent(P>0.05).Basement membrane in colorectal carcinoma showed different degrees of defect，or absent，and the degree related with lymph node metastasis，Dukes staging (P<0.05)，but not with depth of invasion，patients′ gender，age，tumor location and histological type(P>0.05). Conclusion There are closely relationship between tumor angiogenesis，expression of LN and metastasis of colorectal cancer，Dukes stage.The combined detection of VEGF，MVD and LN can determine the invasion and tendency metastasis of colorectal cancer，then estimate the degree of malignant，and give a guide of therapy.