Abstract: Objective To investigate the variation of HE4 concentration in human ovarian carcinoma and its clinicopathologic significance. Methods The serum concentration of HE4 and CA125 in 64 human ovarian carcinoma，40 ovarian benign tumor carried patients and 40 healthy control were detected by ELISA and electrochemistry irradiance methods respectively. Results The mean concentration of HE4 in 64 human ovarian carcinoma，benign tumor and healthy control was(395.2±231.29) pmol/L，(43.86±20.87) pmol/L，and (30.22±9.64) pmol/L respectively.The serum levels of HE4 were significantly higher (P<0.01)in ovarian cancer than those in benign tumor group and healthy control.But there was no significantly difference between ovarian benign tumors and healthy control(P>0.05).It would be best for diagnosis when serum concentration of HE4 was 148.8 pmol/L on ovarian carcinoma.The sensitivity and specificity of diagnosing were 86.4 %and 97.5% respectively.The HE4 assay in receiver operating characteristic-area under the curve (ROC-AUC)was 0.975 (95%CI 0.970~1.0000，P<0.001).There was not significant difference on serum concentration of HE4 in patients with or without lymph node metastasis，ascites and omentum metastasis.The independent factors of ovarian cancer survival was associated with the stage of FIGO，distant metastasis and post-operative residual lesion，but the serous HE4 contain was not independent factors for the prognosis of ovarian cancer. Conclusion It would be helpful to diagnose ovarian cancer on testing of serum HE4 contain.