Abstract: Objective To investigate the expression of miR-106a and miR-24-1 in both colon cancer and adjacent tissues，and analyze the relationship between the expression of miR-106a，miR-24-1 and c-myc gene. Methods To extract RNA from colon cancer tissues and their adjacent ones，detected the mRNA expression of c-myc by RT-PCR.The TaqMan fluorescence quantitative PCR was used to analyze the expression of the miR-106a and miR-24-1 in both colon cancer and adjacent tissues. Results The results showed that the expression of miR-106a in cancer tissues was higher than that in the adjacent tissues in the c-myc positive group（P<0.05），and the expression of miR-106a in cancer tissues was also higher than that in the adjacent tissues in the c-myc negative ones(P<0.05).The expression of miR-24-1 showed no difference either between cancer and adjacent tissues，or between positive and negative c-myc control. Conclusion The expression of miR-106a in both c-myc positive group cancer tissues and the c-myc negative ones were higher than that in adjacent tissues.miR-106a might be a blood screening target of colon cancer.There was no difference of the expression of miR-24-1 in both the c-myc positive group ones and the c-myc negative ones，which need more research to prove the relation between miR-24-1 and colon cancer.