Abstract: Objective To explore the expression and correlation of Survivin and Caspase-3 in pancreatic carcinoma, and to evaluate the correlation of Survivin and Caspase-3 with the clinical pathological features and the survival of patients. Methods Fifty-four cases of pancreatic carcinoma tissues and 14 cases of normal pancreatic tissues were enrolled in our study.All patients were followed up.Expression of Survivin and Caspase-3 were examined by immunohistochemistry in all pancreatic tissues. Results The positive expression of Survivin in pancreatic carcinoma tissues was observed in 85.2% specimens.The positive rate of Survivin expression in normal pancreatic tissues was 35.7%.Meanwhile,the positive rate of Caspase-3 expression was 87.0% and 35.7%, respectively.The expression of Survivin was negatively related with the differentiation degree of pancreatic carcinoma,however,the Caspase-3 expression was positively related with it.The expression of Survivin and Caspase-3 had no correlation with clinical staging.The Survivin expression had correlation with vessel invasion or lymph node metastasis,but not in Caspase-3.The Survivin expression had negative correlation with Caspase-3 expression.The survival rate was significantly different between the patients with negatively and weakly positive Caspase-3 expression and those with moderately and strongly positive expression,but was not significantly different between the patients with different Survivin expression. Conclusion The high expression and negative correlation of Survivin and Caspase-3 in pancreatic carcinoma suggested that Survivin and Caspase-3 proteins probably played a role in onset and progression of pancreatic carcinoma. The expression level of Caspase-3 could be used as an valuable index for predicting the prognosis.