Abstract: Objective To study the effect of multiple stresses on the changes of cellular immunity and tumor marker in oesophageal neoplasia of rats by animal modeling. Methods Wistar rats were chosen for experiment and pathological examination fully confirmed the occurrence of oesophageal cancer in the examined rats.After success of modeling,40 rats with oesophageal neoplasia were randomly divided into two groups,with twenty in each group.One group were treated with chronic composite stress.The other group was used as control,and normally feed.Before the start of experiment,T-lymphocyte subsets,i.e.CD3+,CD4+，CD4+/CD8+ and tumor marker,i.e.CEA,CA19-9,CA724,CYFRA21-1 and SCC in each rat were measured.Four weeks stress was supplied for treated rats.Then above parameters were measured again in the control and treated rats.The data were analyzed between control and treated groups.Therefore,we could conclude the effects of stress on cellular immunity function and tumor markers. Results Values of CD3+,CD4+,CD4+/CD8+ of 40 rats with oesophageal neoplasia before experiment were respectively (65.37±4.51) μg/L,(30.68±5.40) μg/L and (1.37±0.22) μg/L,while they were (82.67±5.55) μg/L,(17.81±1.53) μg/L and (0.84±0.12) μg/L of the treated group after the stress.There were statistic significance before stress and after stress (P＜0.05).In contrast values of the control were (67.14±1.49) μg/L,(35.70±5.72) μg/L and (1.31±0.15) μg/L after experiment,without statistic significance (P＞0.05).There were statistic significance between the control and treatment (P＜0.01).Contents of tumor marker CEA and SCC before experiment were (4.85±0.93) μg/L and (1.82±0.61) μg/L,and after experiment for treated group were (6.82±1.06) μg/L and (3.12±1.21) μg/L with statistic significances compared with the control and before experiment,respectively (P＜0.05).Other three parameters for tumor marker were statistic significance neither the control/treatment,nor before/after experiment (P＞0.05). Conclusion Chronic stress affects the body cell immunity function resulting from immunity decline and impacts the development of tumor.