5-氮杂-2′-脱氧胞苷对肝癌细胞HepG2凋亡及其PEG10基因表达的影响

doi:10.3971/j.issn.1000-8578.2012.01.003

5-氮杂-2′-脱氧胞苷对肝癌细胞HepG2凋亡及其PEG10基因表达的影响

Effects of 5-Aza-2′-deoxycytidine on Apoptosis and Expression of PEG10 Gene in Human Hepato Carcinoma Cell Line HepG2

摘要

摘要: 目的探讨去甲基化药物5-氮杂-2′-脱氧胞苷（5-Aza-CdR）对人肝癌细胞系HepG2凋亡及其PEG10基因表达调控的影响，进一步探讨肝癌的发病机制及5-Aza-CdR治疗肝癌的可行性。方法用浓度为50 μmol/L的5-Aza-CdR分别处理HepG2细胞24、48、72 h，hoechst 33342/PI 双染荧光显微镜检测HepG2细胞凋亡情况。RT-PCR、Western blot法分别检测PEG10 mRNA和蛋白表达水平的变化。结果与对照组相比，5-Aza-CdR作用24、48、72 h后，HepG2细胞的凋亡率明显升高，PEG10 mRNA 和蛋白表达水平显著下降，并呈现时间依赖性（P＜0.05）。结论5-Aza-CdR 可促进HepG2细胞凋亡并抑制PEG10基因的表达。

Abstract: Objective To investigate the effects of demethyhfing agent 5′-Aza-2′-deoxyeytidine(5-Aza-CdR)on the expression of PEG10 gene in human hepato carcinoma cell line HepG2. Methods HepG2 cells were treated with 50.0 μmol/L 5-Aza-CdR for 24,48,72 h.Apoptosis of HepG2 induced by 5-Aza-CdR was identified by fluorescence microscopy with hoechst 33342/PI staining.RT-PCR and Western blot were applied to detect the PEG10 mRNA and protein expression levels,respectively. Results After treated with 5-Aza-CdRat for indicated time,fluorescence microscopy analysis showed that the apoptosis rate of HepG2 cells increased significantly,and the expression of PEG10 mRNA and protein in HepG2 cells were reduced in a time-dependent manner(P＜0.05). Conclusion 5-Aza-CdR can induce HepG2 cell apoptosis and reduce the expression of PEG10 gene.

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