Abstract: ObjectiveTo explore the influence and clinical significance of thermochemotherapy on the expression of CD8+CD28+T (CTL),subtype cells in peripheral blood of patients with advanced lung cancer，colorectal cancer or breast cancer. MethodsAdvanced patients with lung cancer，colorectal cancer or breast cancer (20 cases, respectively) were divided into two groups with thermochemotherapy and chemotherapy randomly.There were 10 coses of lung cancer，colorectal cancer or breast cancer patients were in each group.Thermochemotherapy group was given whole body thermotherapy concurrently with each cycle chemotherapy.Two cycles treatment were given to them.20 healthy volunteers were used as control.The T subtype cells of CD4+，CD8+ and CD8+CD28+ in peripheral blood were analyzed by flow cytometry. ResultsT subtype cells of CD4+，CD8+ and CD4+/CD8+ of the malignant group showed no significance compared with the normal group(P＞0.05)， CD8+CD28+ expressed lower and could be upregulated significantly after thermochemotherapy（P＜0.05）.There were no significance prior and after chemotherapy（P＞0.05）.The response rate and quality of life of thermochemotherapy group were better than those of chemotherapy group(P＜0.05)，3 to 4 grade bone marrow suppression and response of digestive tract showed no significance after treatment in the two groups（P＞0.05）.T subtype cells of CD8+CD28+ showed no correlation with tumor pathological type. ConclusionThe costimulatory molecule of CD28 of T cells expressed lower and existing tumor cells immune escape in advanced malignant patients，thermochemotherapy can upregulate expression of CD8+CD28+ on peripheral T cells,enhance immune surveillance function，showing advantage on curative effect and prognostic compare with chemotherapy.